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KMID : 0525720100150020075
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Journal of Chitin and Chitosan
2010 Volume.15 No. 2 p.75 ~ p.79
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Angiotensin I-Converting Enzyme Inhibitory Activity of Sulfated Chitooligosaccharides with Different Molecular Weights
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Qian Zhong-Ji
Eom Tae-Kil
Ryu Bo-Mi
Kim Se-Kwon
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Abstract
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The angiotensin I converting enzyme (ACE) inhibitory effects of three kinds of sulfated chitooligosaccharides (SCOS),
relatively higher molecular weight SCOS (HMWSCOS, 5-10 kDa), medium molecular weight SCOS (MMWSCOS, 5-3 kDa),
and lower molecular weight SCOS (LMWSCOS, 3-1 kDa), respectively. The MMWSCOS exhibited the higher inhibition
activity with the IC50 value of 0.25 mg/mL than other sulfated chitooligasaccharides (MMWSCOS: 0.775 mg/mL and LMWSCOS: 0.325 mg/ml). Furthermore, all SCOSs showed no cytotoxicity on human embryonic lung fibroblast cell line (MRC-5) and Lineweaver-Burk plots suggest that MMWSCOS and LMWSCOS acts as non-competitive inhibitor to inhibit ACE. Therefore, these results exhibited that substitution of the hydrogen atom at the C-6 position of pyranose residue by the sulfate group promotes ACE inhibitory effects of COS and they would be beneficial ingredients for nutraceuticals and pharmaceuticals against hypertension and related diseases.
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KEYWORD
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angiotensin I converting enzyme, ACE, sulfated chitooligosaccharides, molecular weights INTRODUCTION
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